Table 2.
Summary of NOD-SCID and Rag γC−/− recipient repopulation with CD34+ and CD34− sorted cells from first and second trimester human placenta
| Recipients | Gestation week | Cell number injected | Number repopulated/Number injected | ||
|---|---|---|---|---|---|
| CD34+ | CD34− | CD34+ | CD34− | ||
| NOD-SCID | 6 | 0.6×106 | 1.9×106 | 2/2 | 1/1 |
| Rag γC−/− | 16–17 | 0.6–1.0×106 | 2×106 | 1/3 | 0/4 |
| NOD-SCID | 16–18 | 0.06–0.6×106 | 0.75–1.6×106 | 1/2 | 1/2 |
| NOD-SCID | 19–20 | 0.5×106 | 5–10×106 | 1/1 | 0/2 |
Tissue DNA from all recipients injected with human placenta cells was tested for human engraftment by AMEL PCR. NOD-SCID adult recipients were irradiated with 3 Gy, injected intravenously with the indicated numbers of sorted placenta cells (and 1 × 105 NOD-SCID spleen cells) and analysed for human cell engraftment at 5 weeks post-injection. Rag γC−/− 5 day old recipients were irradiated with 3 Gy, injected in the liver with the indicated numbers of sorted placenta cells (10 μl volume), and at 11 weeks post-injection analyzed for human cell engraftment.