Fig. 5.

Effects of NOX inhibitor on IL-4-induced IL-6 expression. HAEC were pre-treated with the indicated concentrations of apocynin (APO) for 30 min, and then incubated with either PBS (Control) or 10 ng/ml of IL-4 for 4 h (A) or 16 h (B). The mRNA and protein expression levels of IL-6 were determined by real-time RT-PCR (A) and ELISA (B). Values represent the mean ± SEM (n=4). * p < 0.05 vs. Control, # p < 0.05 vs. IL-4.