Fig. 7.
A, MS spectrum of a representative peptide of Pdp2. B, MS spectrum of a phosphopeptide of Pdha1. The top right panel shows both the peptide ion current areas and the corresponding values between the pre- and early diabetic samples. C, the metabolic pathway of the involved proteins that links glycolysis with the tricarboxylic acid cycle. D, Western blot validating the proteomics observation that protein expression of Pdp2 was down-regulated in the development of T2D and that the phosphorylation level of its substrate Pdha1 was coordinately up-regulated. TCA, tricarboxylic acid.