Figure 8.
NG2+ cell growth is not inhibited by CM from normal peritoneal macrophages (PM) or astrocytes (AST). The numbers and sizes of the spheres (A) as well as total cell numbers (B) in the presence of the media conditioned by normal PM or AST were similar to the control group (NG2+ cells alone) after 2 weeks culture. There was reduction of sphere formation and total cell numbers when NG2+ cells were cultured with the OX42+ cell-CM. N = 3. *Significantly different from the NG2+ cells in the absence of conditioned medium. C: Representative cells from sphere culture with OX42+ cell-CM. All cells show co-expression of NG2 and nestin. Scale bar = 100 µm.