FIGURE 3.
Nuclear localization and in situ expression of Pax6(S). A, HEK 293T cells expressing FLAG-Pax6(S) (upper row) or FLAG-Pax6 (lower row) were stained with (from left to right) an anti-Pax6(S) antibody, an anti-FLAG antibody, and a nuclear dye DAPI. Immunofluorescence from these three stainings is overlaid in the rightmost panels. B, Pax6 (left) but not Pax6(S) (right) was detected in the inner nuclear layer (arrow) of the human retina. C, staining of Pax6(S) in 4-month (left) and 5-month (right) embryonic human brain slides is shown. Arrows point out three positive signals. D, staining of Pax6(S) in various human embryonic tissue lysates with Western blot is shown. Bar, 10 μm.