Fig. 8.
Expression of PPARγ, HMMR, Flk-1, FGF2, and caveolin-1 in a lamb model of pulmonary hypertension. PPARγ (A), HMMR (B), Flk-1 (C), FGF2 (D), and caveolin-1 (E) protein levels were determined by Western blot analysis in extracts prepared from peripheral lung tissue of 2-wk old Shunt and control lambs. A representative image is shown for each Western blot. PPARγ protein is significantly decreased in the Shunt lamb (2.7-fold, A), while the protein levels of HHMR (2.8-fold, B), Flk-1 (3-fold, C), FGF2 (2.1-fold, D), and caveolin-1 (4.6-fold, E) were all significantly increased. Data are means ± SE, n = 4, *P < 0.05 vs. control. All protein levels were normalized by reprobing with β-actin.