Fig. 3.
IL-6 mRNA and MCIP1 mRNA levels are increased in C2C12 myotubes by calcium ionophore. A: C2C12 myotubes were treated for 6 h with either 1 mM of the calcium ionophore A23187 or with calcium ionophore and actinomycin D. Actinomycin D treatment completely abolished the significant increase in IL-6 mRNA with calcium ionophore treatment. B: MCIP1 and β-actin mRNA levels in untreated C2C12 myotubes and in C2C12 myotubes treated for 3 or 6 h with ionophore. Ionophore treatment resulted in a significant increase in MCIP1 mRNA levels at 3 h and was still elevated at 6 h of ionophore treatment, while β-actin mRNA levels remained unchanged. *Significantly different from untreated, P < 0.05. C: IL-6 mRNA levels in response to infection of C2C12 myotubes with adenoviral constructs containing either a cytomegalovirus (CMV)-RFP control, a CAMKIIdB, or a calcineurin expression vector. Infection with the calcineurin adenovirus increased IL-6 mRNA levels at ∼500-fold, although this was not significant because of high variability in infectivity. Data are expressed as means ± SE for n = 5 independent experiments.