Fig. 7. Effect of transient sense and antisense eEF1A-1/EF-1α transfection in serum-deprived L6 myotube cultures.
Panel A, viability of differentiated L6 cells after serum deprivation is measured by β-galactosidase activity after transient cotransfection of β-galactosidase reporter vector and the gene of interest. Cells cotransfected with pBK CMV empty vector were used as control (black bars). Cells cotransfected with antisense eEF1A-1/EF-1α in pBK CMV vector are represented by the white bars, and cells cotransfected with the sense eEF1A-1/EF-1α gene in pBK CMV vector by the gray bars. The asterisks represent a significant difference analyzed by Student’s t test (p < 0.001). Panel B, Western blotting of cotransfected cells to show the efficiency of the transfection. The experiment was performed in duplicate from two different cell samples. β-Actin was used as a loading control. Panel C, relative expression of eEF1A-1/EF-1α protein in transfected L6 myotube cultures, including the S.D.