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Wound cells do not produce IL-13. Wound cells were isolated at 1, 3, and 7 days after PVA sponge insertion, incubated in complete medium with Brefeldin A for 6 h, and stained with fluorochrome-conjugated isotype control (left panels) or IL-13-specific (right panels) antibody. Positive control: BALB/c splenocytes cultured as described in Materials and Methods and stimulated with PMA and calcium ionomycin. Results are representative of two experiments.
