Table 1. Genotypes of embryonic and adult mice resultant from mating heterozygous (Tip60+/− × Tip60+/−) mice.
All genotypes were determined by PCR as described in Experimental Procedures. Embryos were at the indicated developmental stages; weaned mice were 1–2 months old. ND = genotypes that could not be determined. Chi-square analysis revealed that the proportion of genotypes at ED2.5 (P = 0.78) and ED3.5 (P = 0.22) did not significantly differ from Mendelian expectations.
Distribution of the Tip60-null Allele in Embryos and Weanlings following Tip60+/− × Tip60+/− Intercrosses.
| ED2.5 | ED3.5 | ED7.5 | ED8.5 | weaned | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| # | % | # | % | # | % | # | % | # | % | |
| +/+ | 15 | 28 | 11 | 16 | 8 | 31 | 32 | 28 | 107 | 34 |
| +/− | 24 | 45 | 39 | 56 | 18 | 69 | 81 | 72 | 207 | 66 |
| −/− | 14 | 27 | 19 | 28 | 0 | 0 | 0 | 0 | 0 | 0 |
| [nd] | [7] | [12] | [4] | [5] | 0 | 0 | 0 | 0 | 0 | 0 |
| totals | 53 | 100 | 69 | 100 | 26 | 100 | 113 | 100 | 314 | 100 |