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. 2009 Dec 11;106(52):22217–22222. doi: 10.1073/pnas.0909773106

Fig. 2.

Fig. 2.

Protein synthesis is impaired in DHX29-silenced cells. (A) Western blot analysis of DHX29 expression in HeLa cells transfected with nontargeting control or DHX29 siRNAs. (B) Effects of DHX29 silencing on protein synthesis. [35S]-methionine labeling of cells transfected with DHX29 or control siRNAs. The y-axis represents counts per minute (cpm) obtained from DHX29-silenced cells divided by cpm obtained from control cells. (C) Expression levels of DHX29 in parental HeLa cells and cells expressing 5 different DHX29-silencing (shRNA 1–5) and nontargeting control (control) shRNAs. (D) [35S]-methionine incorporation in cells expressing nontargeting control or DHX29 shRNA. The y-axis is as in (B).