FIG. 3.
Expression analysis of HCV entry factors in HuH6 cells. (A) Unpermeabilized Huh-7.5, HuH6, or NIH 3T3 cells were stained with CD81-specific antibodies and analyzed by flow cytometry. Filled histograms represent cells stained with the secondary antibody only, whereas open histograms represent staining with the receptor-specific antibodies. (B, C, and D). The total expression of SR-BI (B), occludin (C), or CLDN1 (D) in lysates of NIH 3T3, Huh-7.5, and HuH6 cells was determined by Western blotting.