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Neisserial lipooligosaccharides utilized as substrates in this study. (A) Structures of the core regions of the different LOS substrates. (B) Differences in the lipid A region after different chemical modifications. de-O-Ac, LOS after de-O-acylation by hydrazinolysis; HF-de-O-Ac, de-O-Ac LOS after dephosphorylation by incubation with HF; KOH, LOS after complete deacylation by incubation with KOH and re-N-acetylation.
