Figure 2.
Colocalization of EB1 with cytoplasmic microtubules and the centrosome. Unsynchronized CV-1 cells were fixed in methanol and processed for dual immunofluorescence with antibodies directed against EB1 (GD10) and α-tubulin (YL1/2), in the presence or absence of the microtubule destabilizing drug nocodazole (10 μM). (a, a′, and a′′) Control untreated cells. (b, b′, and b′′) Cells treated with nocodazole for 1 hr and fixed in methanol at −20°C. (c, c′, and c′′) Cells fixed in methanol 5 min after nocodazole removal. Staining was analyzed by confocal microscopy. (Bar, 10 μm.)