Figure 3. Whole tissue expression of 5-HT receptor mRNA transcripts in the anterolateral BNST.
Total RNA was isolated from the anterolateral BNST and reverse transcribed into cDNA. The cDNA was amplified by PCR with primers specific for each of the 5-HT receptors. The PCR products were electrophoresed on a 1% agarose gel. M denotes a molecular weight marker.
