Figure 2.
Heterozygous preCGG KI neurons show significantly reduced dendritic complexity throughout in vitro development. Hippocampal neuronal cultures from heterozygous preCGG mice and WT littermates were fixed weekly after plating. Immunofluorescent labeling was performed using Map2B antibody. (A) Representative images from the two different genotypes at three time points. (B and C) Quantification of dendrite length and the number of branch points of individual neurons, respectively, calculated with Imaris FilamentTracer software as described in Materials and Methods. The heterozygous preCGG neurons show delayed dendritic growth from an early developmental stage (7 DIV) throughout their maturation (21 DIV). N = 3 separate cultures with 30–38 randomly selected neurons for each time points for each genotype. Scale bar: 20 µm.