Table 2.
Sequences of Primers and Probes for Q-RT-PCR Analyses of mRNA Levels*
| mRNA levels | Sequence |
|---|---|
| PLCβ1 | Forward primer: 5′–TGCACGCCTTGCAACTCA–3′ |
| Reverse primer: 5′–CATCCCACTTGACGAATTTGG–3′ | |
| Probe: 5′–FAM - CCGTGTGCGTGTCCGACAGCC-BHQ–3′ | |
| PLCβ2 | Forward primer: 5′–CTGCCCTCGGTGCTTGTC–3′ |
| Reverse primer: 5′–TTGTACACGGATTCTGGGAAGTC–3′ | |
| Probe: 5′– FAM - TTGCAAAAGGCAAAAATGATGCTATCAACC-BHQ–3′ | |
| PLCβ3 | Forward primer: 5′–GAACGCCTCCCGGAACA–3′ |
| Reverse primer: 5′–ATCCGTCCATCCTGGTTCAC–3′ | |
| Probe: 5′–FAM - CTTCCTGCGGAAAGCATACACGAAGC–BHQ–3′ |
*
Primers and probes were designed by using Applied Biosystems’ Primer Express 2.0 software and synthesized at the University of Medicine and Dentistry of New Jersey Molecular Resource Facility. 5′-endsof the probes were labeled with the fluorescent dye 6-carboxy fluorescene (FAM), and 3′-ends were coupled to the quencher molecule, Black HoleQuencherTM dye-1 (BHQ-1).