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. Author manuscript; available in PMC: 2010 Dec 11.
Published in final edited form as: Vaccine. 2009 Oct 13;28(2):494–511. doi: 10.1016/j.vaccine.2009.09.133

Table 1.

PCR primers used to amplify VACV to clone into VEE replicon vectors

Source of
VACV gene		 5’ Forward primer 3’ 5’ Reverse primer 3’
pWRG/A33R GCTCTAGAATGATGACACCAGAAAACGACG GCTCTAGATTAGTTCATTGTTTTAACAC
pWRG/A27L GCTCTAGAATGGACGGAACTCTTTTCCCCG GCTCTAGATTACTCATATGGACGCCGTCCAG
pWRG/B5R GCTCTAGAATGAAAACGATTTCCGTTGTTAC GCTCTAGATTACGGTAGCAATTTATGGAAC
pWRG/L1R GCTCTAGAATGGGTGCCGCAGCAAGCATAC GCTCTAGAATCAGTTTTGCATATCCGTGGTAG
pWRG/tPA-
L1R		 GTCTAGAATGGATGCAATGAAGAGAGGG CTCTAGACTCAGTTTTGCATATCCGTGGTAGC