Figure 3.
Final purification and identification of DCs. (A) Final purification of DC1 was performed according to the scheme shown. Mono-S fractions were assayed for DC1 activity in the presence of P1.0, rDSIF, and crude DC2 (mono-Q #14). The same fractions were analyzed by SDS-PAGE and silver staining. Proteins in an active fraction were also visualized by Coomassie staining and were subjected to LC-MS/MS analysis. (B) Final purification of DC2 was performed according to the scheme shown. Fractions derived from the second mono-Q column were assayed for DC2 activity in the presence of P1.0, rDSIF, and crude DC1 (mono-Q #12). Proteins were analyzed as described in A.