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Separation of DC activity into two components. (A) Scheme for partial purification of DCs. (B–D) D.3-derived mono-Q fractions were assayed for DC activity. In B, P1.0, rDSIF, and one of the column fractions were used as protein source. An anti-Spt5 immunoblot is shown below. In C and D, the same mono-Q fractions were assayed in the presence of an additional mono-Q fraction.
