Fig. 9.

Reduced half-life of p33 in TKY848 yeast strain. (A) Translation of mRNAs was stopped in yeast with cyclohexamide, followed by Western blot analysis of p33 levels with anti-His antibody at various time points. Strain TKY102 expressing WT eEF1A was used as a control. (B) Calculation of the half-life of p33 in TKY848 and TKY102. The experiments were repeated and the standard deviation is shown.