TABLE 1.
Candidate survival genes
Our siRNA screen identified 55 survival genes that significantly induced glioma cell death when suppressed. Classification based on shared molecular and biological function was used to subdivide these genes by shared molecular and biological functions, and the subdivisions were further ordered by increasing cell viability. Cell viabilities were calculated by normalizing data from siRNA targeting wells to in-plate negative controls.
| Gene Symbol | Gene Name | Viability |
|---|---|---|
| % | ||
| Ion Channels | ||
| CLCN3 | Chloride channel 3 | 22.20 |
| Transcription/translation regulators | ||
| POLR2F | Polymerase (RNA) II (DNA directed) polypeptide F | 9.66 |
| POLR2A | Polymerase (RNA) II (DNA directed) polypeptide A, 220 kDa | 24.82 |
| CGI-04 | Tyrosyl-tRNA synthetase 2 (mitochondrial) | 27.61 |
| POLR2G | Polymerase (RNA) II (DNA directed) polypeptide G | 30.81 |
| Receptors | ||
| TLR2 | Toll-like receptor 2 | 15.52 |
| ITGB3 | Integrin, β 3 (platelet glycoprotein IIIa, antigen CD61) | 20.75 |
| Transporters | ||
| SLC7A2 | Solute carrier family 7 (cationic amino acid transporter, y+ system), member 2 | 23.17 |
| Hydrolase | ||
| GALNS | Galactosamine (N-acetyl)-6-sulfate sulfatase (Morquio Syndrome, mucopolysaccharidosis type IVA) | 25.30 |
| Cytoskeletal protein or associated | ||
| TUBGCP6 | Tubulin, γ -complex associated protein 6 | 13.45 |
| MPHOSPH1 | M-phase phosphoprotein 1 | 26.04 |
| Protease | ||
| PSMC3 | Proteasome (prosome, macropain) 26S subunit, ATPase, 3 | 8.35 |
| PSMD14 | Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 | 10.90 |
| PSMC6 | Proteasome (prosome, macropain) 26S subunit, ATPase, 6 | 12.17 |
| PSMC5 | Proteasome (prosome, macropain) 26S subunit, ATPase, 5 | 17.05 |
| PSMB2 | Proteasome (prosome, macropain) subunit, β type, 2 | 26.70 |
| PSMB4 | Proteasome (prosome, macropain) subunit, β type, 4 | 30.56 |
| PSMA6 | Proteasome (prosome, macropain) subunit, α type, 6 | 30.62 |
| PSMA4 | Proteasome (prosome, macropain) subunit, α type, 4 | 31.58 |
| PSMA3 | Proteasome (prosome, macropain) subunit, α type, 3 | 33.00 |
| PSMA5 | Proteasome (prosome, macropain) subunit, α type, 5 | 33.87 |
| PSMA2 | Proteasome (prosome, macropain) subunit, α type, 2 | 34.50 |
| PSMA1 | Proteasome (prosome, macropain) subunit, α type, 1 | 34.72 |
| Ligase | ||
| HLCS | holocarboxylase synthetase (biotin-[propionyl-Coenzyme A-carboxylase (ATP-hydrolyzing)] ligase) | 12.48 |
| Transferase | ||
| SIAT7E | ST6 (α -N-acetyl-neuraminyl-2,3-β -galactosyl-1,3)-N-acetylgalactosaminide α -2,6-sialyltransferase 5 | 11.16 |
| SIAT4B | ST3 β -galactoside α -2,3-sialyltransferase 2 | 11.25 |
| SPTLC1 | Serine palmitoyltransferase, long chain base subunit 1 | 11.66 |
| POMT1 | Protein-O-mannosyltransferase 1 | 19.39 |
| PIGQ | Phosphatidylinositol glycan, class Q | 20.85 |
| Signaling molecules | ||
| CHN1 | Chimerin (chimaerin) 1 | 15.67 |
| ARHGEF11 | Rho guanine nucleotide exchange factor (GEF) 11 | 24.52 |
| RAS family proteins | ||
| RAN | RAN, member RAS oncogene family | 5.84 |
| RAB40B | RAB40B, member RAS oncogene family | 29.68 |
| Kinases | ||
| K-ALPHA-1 | K-ALPHA-1 | 8.22 |
| BUB1B | BUB1 budding uninhibited by benzimidazoles 1 homolog β (yeast) | 13.49 |
| PRKCD | Protein kinase C δ | 15.55 |
| AKT3 | v-akt murine thymoma viral oncogene homolog 3 (protein kinase B γ ) | 16.28 |
| TNNI3K | TNNI3 interacting kinase | 20.08 |
| Oxygenase | ||
| CYP1B1 | Cytochrome P450, family 1, subfamily B, polypeptide 1 | 32.29 |
| Unclassified | ||
| BTN2A3 | Butyrophilin, subfamily 2, member A3 | 11.45 |
| UBL5 | Ubiquitin-like 5 | 19.35 |
| ZAP128 | ZAP128 | 20.51 |
| GRCC9 | GRCC9 | 26.60 |
| GRIN1 | Glutamate receptor, ionotropic, N-methyl d-aspartate 1 | 29.05 |
| PKD1L3 | Polycystic kidney disease 1-like 3 | 30.47 |
| Other | ||
| DDX39 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 39 | 6.90 |
| SPINK1 | Serine protease inhibitor, Kazal type 1 | 11.19 |
| GRHPR | Glyoxylate reductase/hydroxypyruvate reductase | 14.21 |
| IDH3B | Isocitrate dehydrogenase 3 (NAD+) β | 19.42 |
| COX7A2 | Cytochrome c oxidase subunit VIIa polypeptide 2 (liver) | 20.23 |
| BST1 | Bone marrow stromal cell antigen 1 | 21.57 |
| RRM1 | Ribonucleotide reductase M1 polypeptide | 21.76 |
| GPX2 | Glutathione peroxidase 2 (gastrointestinal) | 22.70 |
| SMS | Spermine synthase | 24.90 |
| GUSB | Glucuronidase β | 29.93 |