Figure 7.
(A) Structure of the 2-site ELISA for detection of antibodies against the HIRMAb-IDUA fusion protein. The HIRMAb-IDUA fusion protein is used as the capture reagent, and the biotinylated HIRMAb-IDUA fusion protein is used as the detector reagent, along with a complex of streptavidin (SA) and horseradish peroxidase (HRP); the biotin moiety is designated, ‘B’. (B) Absorbance at various dilutions of a rabbit antiserum prepared against the HIRMAb-IDUA fusion protein (diamonds), and the respective pre-immune serum (squares). Data are means of duplicates that varied <10%. (C) Absorbance at 1:50 dilutions of Rhesus monkey plasma taken at day 28, following the administration of 0, 0.2, 2, or 20 mg/kg of the HIRMAb-IDUA fusion protein twice/week for 4 consecutive weeks of the study. A significant immune reaction is registered with an (A490–A655) signal >0.3.