Table 2.
Primary antibodies
| Antibody | Host | Dilution factor | Source |
| LHX2 | Rabbit | 50 | Edwin Monuki |
| LHX2 | Goat | 1000 | Santa Cruz (sc-19344) |
| VSX2 | Sheep | 300 | Exalpha Biologicals (X1180P) |
| PAX6 | Mouse | 10 | DSHB (P3U1) |
| PAX6 | Rabbit | 1500 | Chemicon (AB2237) |
| SHH | Mouse | 50 | DSHB (5E1) |
| PAX2 | Rabbit | 100 | Covance (PRB-276P) |
| OTX2/1 | Rabbit | 1000 | Chemicon (AB9566) |
| SIX3 | Rabbit | 800 | Guillermo Oliver |
| SOX2 | Rabbit | 400 | Abcam (ab15830) |
| MITF | Mouse | 400 | Exalpha Biologicals (X1405M) |
| pSMAD1/5/8 | Rabbit | 500 | Cell Signaling Technology (9511S) |
| pERK1/2 | Rabbit | 500 | Cell Signaling Technology (9101S) |
| pHH3 | Rabbit | 500 | Upstate Biotechnology (06-570) |
| β-gal | Rat | 1000 | Nadean Brown |