Figure 4.

Anticipated results for the G40P case. Tilted pairs of digitized micrographs were processed to yield a data set of 14,000 particle pairs. The untilted particles were classified in threefold and sixfold symmetric particles based on their rotational spectra. For both classes, the untilted particles were aligned using maximum-likelihood refinement, and the tilted particles were used to calculate the corresponding random conical tilt reconstructions.