Figure 7.
CK2β increases ALK-1-mediated and TGFβ1-and BMP-9-stimulated inhibition of endothelial cell migration. HMEC-1 cells were nucleofected with ALK-QD (A), ALK-1 (B–D), and CK2β (B–D), as indicated. Twenty-four hours later, cells were trypsinized and counted. Equal numbers of cells were plated on transwells coated with fibronectin and treated with the indicated amounts of TGFβ1 (B) or BMP-9 (C, D) and assessed for migration 12 h later. Cells on the underside of the transwell filter were fixed, stained for their nuclei, and counted (A, C, D). Numbers of migrated cells were counted using Image J software, normalized to HMEC-1 cells, and graphed. Panel B shows sample images of HMEC-1 cells that migrated under the indicated conditions in the presence of BMP-9. Data are means of 3 independent experiments; error bars = sd.