Fig. 3.
Egr1 control regions, TCF ADs, and TCF transcript abundance. (A) Serum response elements (SREs) within the Egr1 promoter are marked by gray rectangles. The +1 indicates the transcription start site relative to these elements. The TATA box is shown in white. (B) Alignment of the three TCF ADs. Identical residues are marked by black rectangles. Amino acid residues exclusive to SAP and NET are marked by gray rectangles. Arrows indicate conserved serine residues needed for activation, and asterisks mark hydrophobic resides that disrupt function when mutated. (C) TCF mRNA abundance determined by qRT-PCR shown as copies per cell for Elk1 (black), Sap1 (white), and Net (gray) RNAs in WT and KO cells. Mean values from three independent determinations are listed at the bottom. Error bars indicate SD. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.