FIG. 1.
Bioreactor design. (A) Schematic presentation of the bioreactor system used to provide perfusion through six cell-seeded scaffolds that were press-fit into the culture wells. Scaffolds were 4-mm-diameter × 4-mm-high plugs of fully decellularized, mineralized bone. (B) Computational Fluid Dynamics simulation of flow through channels indicating uniform flow to all six wells. (C) Complete experimental set-up showing bioreactor, perfusion loop, and peristaltic pump. (D) Bioreactor on a microscope stage during imaging of tissue constructs in situ. Color images available online at www.liebertonline.com/ten.