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. Author manuscript; available in PMC: 2010 Mar 4.
Published in final edited form as: Cell. 2009 Sep 4;138(5):1005–1018. doi: 10.1016/j.cell.2009.06.023

Figure 5. cebp-1 protein and mRNA are localized in touch neuron axon and synapses.

Figure 5

(A) Expression of functional GFP::CEBP-1 in touch neurons. GFP is present along the axon (left panel), in the cell body (middle) and synapse (right). (B) GFP::NLS::MS2 reporter detects mRNA of cebp-1 in touch neuron axons. Illustrations of the constructs are shown above each image. Axonal fluorescence intensity as a fraction of peak cell body intensity is plotted versus distance along the axon from the cell body. GFP::NLS::MS2 alone (juEx1982) is concentrated in the cell body, and faintly seen along the axon. Coexpression of juEx1982 with cebp-1 transgenes containing six MS2 binding sites in the cebp-1 3′ UTR (juEx1984), but not in unc-54 3′ UTR (juEx2013), results in increased punctate GFP in axons. Axonal expression of GFP::NLS::MS2 + CEBP-1-cebp-1 3′ UTR is decreased in dlk-1(lf) animals, but significantly elevated in rpm-1(lf). (C) Average GFP::NLS::MS2 fluorescence intensity in axons when co-expressed with juEx1984 as % of cell body intensity (n = 15 per genotype). Data shown as mean ± SEM; statistics, Anova. Scale, 10 μm.