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. 2009 Oct 13;6:90. doi: 10.1186/1742-4690-6-90

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Copyright © 2009 Hoque et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Drug effects on luciferase expression from an HIV-1 molecular clone. A. Comparison of drug effects on luciferase expression from the pNL4-3-LucE^-molecular clone in 293T cells. The molecular clone pNL4-3-LucE^-and pCMV-Ren were transfected into 293T cells. Drugs were added where indicated at the following concentrations: P2 (30 μM), CPX (30 μM), DEF (250 μM), or DFOX (15 μM). Dual luciferase assays were conducted at 12 hr post-transfection. Firefly (FF) luciferase expression was normalized to Renilla luciferase (Ren) from pCMV-Ren (mean of 2 experiments in duplicate, ± SD). B. Expression in Jurkat cells was assayed essentially as in panel A. C. Firefly and Renilla luciferase RNA expression was analyzed in 293T cells treated as in panel A by RPA using ³²P- [UTP] labeled antisense RNA probes corresponding to the C-termini of the FF and Ren luciferase mRNAs. D. Comparison of drug effects on p24 expression from the pNL4-3-LucE^-molecular clone in 293T cells. Drugs were added where indicated to the same concentrations as in A. p24 levels were determined in cell extract at 12 hr post-transfection.