Figure 5.
(A) The steady-state level of Ho-LacZ is compared with that of LacZ by the ONPG assay in mutants of the SCF, skp1, cdc53, cdc4, met30, and their isogenic wild types. Fb1 is Δyml088w, Fb2 is Δynl311c, Fb3 is Δyjl149w, and Fb4 is Δyor080w. Ho is stabilized in skp1, cdc53, and the Δyml088w F-box mutant. (B) Gel with accompanying graph showing degradation of Ho in cdc53 and skp1 mutants compared with their congenic wild type. (C) Degradation of Ho is retarded in a strain deleted for the F-box ORF YML088w compared with its isogenic parent.