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. Author manuscript; available in PMC: 2009 Oct 28.
Published in final edited form as: J Vasc Res. 2007 Dec 19;45(3):222–232. doi: 10.1159/000112544

Fig. 4.

Fig. 4

pGlcNAc-induced cell motility is integrin dependent. a Quantitation of the number of cells migrated through transwells coated with fibronectin after pretreatment with antibodies (ab) directed against αVβ3 or α5β1 (CD49e) for 15 min prior to stimulation with pGlcNAc (NAG, 10 μg/ml). SS = Serum-starved EC. b As in a, except the transwells were coated with vitronectin. Each assay was performed in triplicate, 3 independent times. c Western blot analysis of total protein isolated from serum-starved EC (SS) treated with pGlcNAc (NAG, 10 μg/ml) for the times indicated. Blots were probed with antibodies (ab) against phosphorylated FAK (P-FAK) and the p85 subunit of PI3 kinase as a loading control.