Fig. 2.
The JAK-STAT pathway modulates mosquito susceptibility to dengue infection. Dengue titers in the midguts of PIAS dsRNA-, hop dsRNA-, dome dsRNA-, and GFP dsRNA-injected mosquitoes were determined by plaque assays in C6/36 cells at 3 (A) or 7 (B) days after feeding on DENV-2-infected blood. The significance of gene silencing effect on DENV-2 loads in experimental samples, compared with dsGFP-treated controls, was determined by Student's t test. *, P < 0.05. Standard deviation bars for four biological replicates are shown.