TABLE 3.
Open reading frames identified in pEK516
| Gene name | Nucleotide positiona | Function encoded |
|---|---|---|
| tnpA | Compl. 34-2931 | Transposase of Tn501 |
| tnpR | 3023-3630 | Resolvase of Tn21 |
| tnpA | 3627-4473 | Transposase of Tn1721 |
| pecM | 4938-5823 | Hypothetical protein |
| tet(A) | Compl. 5853-7128 | Tetracycline resistance protein, class A |
| tet(R) | 7132-7809 | Tetracycline resistance regulator protein |
| tnpA | Compl. 8124-9714 | Transposase of Tn1721 |
| tnpA | 9705-9980 | NH-2 terminal of the transposase of IS26, pseudogene |
| tnpA | 9980-10663 | COOH− terminal of the transposase of IS26, pseudogene |
| aac6′-Ib-cr | 10669-11361 | Aminoglycoside N(6′)- acetyltransferase |
| blaOXA-1 | 11447-12322 | β-Lactamase OXA-1 precursor |
| catB4 | 12460-13008 | Chloramphenicol acetyltransferase |
| tnpA | Compl. 12954-13658 | Transposase of IS26 |
| aacC3 | 13765-14625 | Aminoglycoside N(3′)-acetyltransferase III |
| BASYS0001 | 14638-15180 | Hypothetical protein |
| BASYS000006 | 15251-15583 | Hypothetical protein |
| insFI | 15580-16428 | Putative transposase of IS986/IS6110 |
| tnpA | Compl. 16374-17078 | Transposase of IS26 |
| tnpA | Compl. 17125-19140 | Transposase of Tn3, truncated |
| amb | 19400-19732 | Hypothetical protein |
| blaCTX-M-15 | Compl. 19779-20654 | Extended-spectrum β-lactamase CTX-M-15 |
| tnpA | Compl. 20910-22172 | Transposase ISEcp1 |
| tnpA | Compl. 22354-22731 | Transposase of Tn3 |
| tnpR | 22700-23293 | Resolvase of Tn3 |
| blaTEM-1 | 23476-24336 | β-Lactamase TEM precursor |
| tnpA | Compl. 2447-26954 | Transposase of Tn21 |
| tnpA | Compl. 27097-27801 | Transposase of IS26 |
| repA1 | Compl. 28926-29795 | Replication initiation protein RepA1 of the FII replicon |
| repA4 | Compl. 30088-30342 | Regulator of repA1 expression, FII replicon |
| yihA | Compl. 30582-31172 | Hypothetical protein |
| hha | Compl. 31210-31419 | Hemolysin expression-modulating protein |
| yigB | Compl. 31465-31938 | Putative endonuclease precursor |
| insB2 | Compl. 31971-32474 | Insertion element IS1 protein InsB |
| yfhA | Compl. 32697-33119 | Hypothetical protein |
| traR | Compl. 33112-33333 | Conjugal transfer protein |
| traV | Compl. 33468-33983 | F pilus assembly |
| trbD | Compl. 33980-34300 | Conjugal transfer protein |
| traP | Compl. 34287-34874 | Conjugal transfer protein |
| traB | Compl. 34843-36294 | F pilin assembly |
| traK | Compl. 36294-37022 | F pilin assembly |
| traE | Compl. 37009-37575 | F pilin assembly |
| traL | Compl. 37597-37908 | F pilin assembly |
| traA | Compl. 37923-38282 | F pilin subunit |
| traY | Compl. 38315-38542 | oriT nicking |
| traJ | Compl. 38679-39350 | Regulation |
| traM | Compl. 39544-40029 | Mating signal |
| gene X (orf39) | 40250-40852 | X− polypeptide; transglycosylation |
| yubP | Compl. 41149-41970 | Hypothetical protein |
| yeiA | Compl. 42081-42377 | Hypothetical protein |
| yehA | 42677-43054 | Hypothetical protein |
| tnpA | Compl. 43058-44461 | Transposase of ISPsy5 |
| ECs1338 | Compl. 44702-45052 | Hypothetical protein |
| ECs1337 | Compl. 45049-45474 | Hypothetical protein |
| hok | Compl. 45832-45990 | Postsegregational killing protein Hok |
| mok | Compl. 45836-46048 | Modulator of Hok protein, Mok |
| psiA | Compl. 46270-46989 | Plasmid SOS inhibition protein A |
| psiB | Compl. 46986-47423 | Plasmid SOS inhibition protein B |
| yefA | Compl. 47489-48064 | ParB-like nuclease |
| parB | Compl. 48091-49512 | ParB-like partitioning protein |
| BASYS00066 | 49537-49884 | Hypothetical protein |
| ydeA | Compl. 49573-49812 | Hypothetical protein |
| ssb | Compl. 49864-50430 | Single-strand binding protein |
| yddA | 50693-51154 | Hypothetical protein |
| ydcA | Compl. 51240-51803 | Hypothetical protein |
| ydbA | Compl. 51850-53211 | Hypothetical protein |
| SC | Compl. 54531-54722 | Hypothetical protein |
| ycjA | Compl. 54719-55141 | Hypothetical protein |
| yicB | Compl. 55188-55613 | Antirestriction protein KlcA |
| ychA | Compl. 56031-56858 | Hypothetical protein |
| ycgB | Compl. 56858-57292 | Hypothetical protein |
| P030 | Compl. 57306-57527 | Hypothetical protein |
| ycgA | Compl. 57528-58175 | Hypothetical protein |
| parM | 58728-59690 | Plasmid segregation protein |
| stbB | 59690-60043 | Stable plasmid inheritance protein |
| ycdB | Compl. 60166-60447 | Hypothetical protein |
| ycdA | 60476-60712 | Hypothetical protein |
| tnpA | 62389-63093 | Transposase of IS26 |
| tir | 63133-63684 | Inner-membrane protein |
| pemI | 63777-64034 | Stable plasmid inheritance, antitoxin |
| pemK | 63967-64368 | Stable plasmid inheritance, toxin |
a
Compl., gene is the reverse complement of the positions shown.