Skip to main content
. 2009 Jul 13;53(10):4138–4146. doi: 10.1128/AAC.00162-09

FIG. 2.

FIG. 2.

Analysis of LAM from M. smegmatis recombinant strains expressing mutant alleles of EmbC. Recombinant strains carried single-copy integrated plasmids expressing EmbC alleles from the Ag85a promoter. LAM was extracted from M. smegmatis and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (on a denaturing, nonreducing 16% acrylamide gel) and periodic acid-Schiff staining (A) and by Western blotting using the anti-LAM antibody CS-35 (B). Lane 1, wild-type M. smegmatis; lane 2, the uncomplemented M. smegmatis embC deletion mutant; lanes 3 to 8, the M. smegmatis embC deletion mutant carrying different embCMt alleles: wild type (lane 3), M300L (lane 4), M300I (lane 5), M300V (lane 6), T270I (lane 7), and D294G (lane 8).