. Author manuscript; available in PMC: 2010 Oct 30.

Published in final edited form as: J Mol Biol. 2009 Aug 26;393(3):586–597. doi: 10.1016/j.jmb.2009.08.050

Table 1.

Amplitude of ATP-induced distance changes in α-ddm micelles.

Mutant	Location	Experimental Distance R_av (Å), detergent			Calculated Distance R_Cα-Cα (Å), MsbA,
Mutant	Location	Apo	ADP-Vi	ΔR_av	Open Apo	AMPPNP	Δ R_Cα-Cα	Closed Apo
43	TM1	35±2.5	38±3	−3	33	31	2	27
42		36±10	36±12	0	31	28	3	32
28		>70	53±4.25	> 17	64	43	21	52
24		>70	55±1.55	> 15	71	44	27	52
104^†	TM2	55±4.5	28±2.75	27	67	29	38	36
106 ^†		>70	47±2	>23	76	33	43	42
110		>70	50±1.75	>20	77	37	40	44
115	IH1	51±3.5	32±1	19	62	31	31	28
116		57±6.5	26±6.7	31	59	24	35	26
118		45±6	41±3	4	51	29	22	17
120		47±10.5	31±2	16	47	18	29	8
121	TM3	51±10			51	13	38	12
139		62±17.5	30±1.25	32	51	26	25	30
141 ^†		33±5.5	20±1.5	13	40	18	22	24
142^*		54±7	30±7.5	24	45	21	24	30
143^*		54±5.6	26±1.5	28	47	28	19	33
144^†		35±2.5	20±2.25	15	41	25	16	27
146		42±3.75	37±3.5	5	48	27	21	37
158		36±1.5	51±7.5	−15	41	46	−5	43
162	EL2	44±3.25	51±6.5	−7	39	53	−14	47
166	EL2	51±6.5	>65	> −14	35	54	−19	49
167	TM4	37±6.5	51±11	−14	34	48	−14	47
183		43±13.25	53±4	−10	38	44	−6	42
187		46±6.25	51±6	−5	41	41	0	39
394 ^*	NBD	>70	55±4	>15	82	48	34	31
482			60±0.5		73	22	51	52
561		>70	42±1.5	>28	86	36	50	37

analyzed by Gaussian fit, otherwise by Tikhonov fit.

^†

bimodal distance distributions

cytoplasm Inline graphic extracellular