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. Author manuscript; available in PMC: 2010 Oct 1.

Published in final edited form as: J Surg Res. 2009 Mar 4;156(2):257–264. doi: 10.1016/j.jss.2009.02.005

FIG. 4 — Effects of HIV Nef on eNOS mRNA and NO levels in HPAECs. HPAECs were treated with Nef in a concentration-dependent manner, and eNOS mRNA levels were measured with real-time PCR. (A). The eNOS mRNA levels in HPAECs were significantly decreased in Nef-treated cells in a concentration-dependent manner, compared with controls. n = 4. (B). NO release from HPAECs was determined by measuring the culture medium for the accumulation of its stable degradation products, nitrite and nitrate using Griess assay method. Nef substantially decreased NO release compared with controls (n = 4). *P < 0.05 versus controls (medium only).

FIG. 4 — Effects of HIV Nef on eNOS mRNA and NO levels in HPAECs. HPAECs were treated with Nef in a concentration-dependent manner, and eNOS mRNA levels were measured with real-time PCR. (A). The eNOS mRNA levels in HPAECs were significantly decreased in Nef-treated cells in a concentration-dependent manner, compared with controls. n = 4. (B). NO release from HPAECs was determined by measuring the culture medium for the accumulation of its stable degradation products, nitrite and nitrate using Griess assay method. Nef substantially decreased NO release compared with controls (n = 4). *P < 0.05 versus controls (medium only).