Figure 3. Preeclamptic MPs contain more DNA.
MPs were quantitated by flow cytometry using a fluorescent bead assay described in Materials and Methods. Preeclamptic (n = 10) and normotensive (n = 9) plasma samples were labeled with MEM-G/1, or IgG1 isotype control mAbs, stained with PicoGreen (dsDNA dye) and analyzed by flow cytometry. A total of 10,000 events were taken. The y-axis of the box plots represents the mean fluorescent intensity of DNA per HLA-G+ MPs from preeclamptic and normal pregnancies. Closed triangles = medians; diamonds = 1st quartile; circles = 3rd quartile; open triangles = max; squares = min. Asterisk (*) indicates significant difference (p < 0.05) compared to non-pregnant control samples.
