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. 2009 Sep 28;206(10):2191–2204. doi: 10.1084/jem.20091480

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© 2009 Tachdjian et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — STAT6-dependent and -independent effects of the R576 substitution. (A and B) Induction of MUC5AC expression in airway epithelial cells. (A) IL-13tg⁺ B6.Il4ra^Q576/Q576 and B6.129Il4ra^R576/R576 mice, and tg-negative controls, that were either STAT6 sufficient or deficient were examined by immunofluorescence staining for expression of MUC5AC after doxycycline (Dox) treatment (red-staining cells). (B) MUC5AC⁺ cells (red-staining cells; A) were counted and expressed as the percentage of total airway epithelial cells (DAPI [blue]-staining nuclei) in the respective airways. (C and D) Lung tissue eosinophilia. (C) Mouse groups as in A were treated with doxycycline, and their lungs were stained with anti-eosinophil MBP1 antibodies (red-staining cells). (D) MBP1⁺ cells were counted and expressed as mean cell numbers per 20× objective field (n = 10 fields per group). (E and F) Real-time PCR analysis of Ccl11 and Alox15 expression in the different mouse groups treated with doxycycline. Values represent means ± SEM of 4–15 mice per group derived from three to five independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Bars, 100 µm.