TABLE 1.
Primers used for real time quantitative PCR
| Gene | Forward | Reverse |
|---|---|---|
| ASNS mRNA | 5′-GCAGCTGAAAGAAGCCCAAGT-3′ | 5′-TGTCTTCCATGCCAATTGCA-3′ |
| ASNS transcription activity | 5′-CCTGCCATTTTAAGCCATTTTGC-3′ | 5′-TGGGCTGCATTTGCCATCATT-3′ |
| CHOP mRNA | 5′-CATCACCACACCTGAAAGCA-3′ | 5′-TCAGCTGCCATCTCTGCA-3′ |
| C/EBPβ mRNA | 5′-CGCAACCCACGTGTAACTGTCAG-3′ | 5′-CAGCAACAAGCCCGTAGGAACA-3′ |
| SNAT2 mRNA | 5′-GTGTCCTGTGGAAGCTGCTTTGA-3′ | 5′-CAGGTACAAGAGCTGTTGGCTGTGT-3′ |
| ATF3 mRNA | 5′-TGATGCTTCAACACCCAGGCC-3′ | 5′-AGGGGACGATGGCAGAAGCA-3′ |
| ATF4 mRNA, coding region | 5′-GGGACAGATTGGATGTTGGAGA-3′ | 5′-ACCCAACAGGGCATCCAAGT-3′ |
| ATF4 mRNA, 3′-untranslated region | 5′-TTGAGGATAGTCAGGAGCGT-3′ | 5′-TGGAACACACAGCTACAGCA-3′ |
| glyceraldehyde-3-phosphate dehydrogenase mRNA | 5′-TTGGTATCGTGGAAGGACTC-3′ | 5′-ACAGTCTTCTGGGTGGCAGT-3′ |
| ASNS, promoter ChIP | 5′-TGGTTGGTCCTCGCAGGCAT-3′ | 5′-CGCTTATACCGACCTGGCTCCT-3′ |
| ASNS, Exon 7 ChIP | 5′-GCAGCTGAAAGAAGCCCAAGT-3′ | 5′-TGTCTTCCATGCCAATTGCA-3′ |