Figure 7.
Absence of autoimmunity upon systemic guanylyl cyclase C (GCCECD) immunization. A) Serum antinuclear antibody (ANA) quantification from naive, LacZ-adenovirus (AV)–, or GCCECD-AV–immunized C57BL/6 or naive MRL/MpJ-Faslpr/J mice (positive control). Data represent mean serum ANA concentrations and upper 95% confidence intervals. B) Histopathologic analysis of tissues collected from BALB/c mice immunized with control or GCCECD-AV or control or GCCECD-ARV (sequential AV, rabies, and vaccinia virus from Figure 2, C–F). Images are representative sections from n = 3 mice per immunization group. Bar = 200 μm.