Table 1. Mitotic indices in wild-type and grp embryos.
| Percentage of embryos in M phase (SD/n) | Percentage of embryos in M phase before chromosome segregation (SD) | Percentage of embryos in M phase after chromosome segregation (SD) | n | |
|---|---|---|---|---|
| Wild type | 58 (6/304) | 60 (3) | 40 (3) | 178 |
| grp1/grp1 | 64 (5/167) | 77 (3) | 23 (3) | 110 |
| grp1/Df | 60 (7/111) | 73 (5) | 27 (5) | 71 |
Syncytial embryos were fixed and stained for DNA, to determine the mitotic stage according to Edgar et al. [6]. Division cycle was determined as in Figure 2 legend. Mitotic indices are from cycles 1-11. Chromosome segregation data were from cycles 1-9 but the samples contained embryos mainly in cycles 6-9; before chromosome segregation includes prophase, prometaphase and metaphase; after segregation includes anaphase and telophase (Au: OK?). As described previously, chromosomes do not condense properly onto the metaphase plate in grp embryos [3], even before cycle 9 (data not shown); consequently, it is harder to distinguish late prophase from metaphase in grp embryos, and hence our division of mitotic stages into before and after chromosome segregation (Au: OK?). The data were from 3 and 5 different experiments, respectively, for grp and wild type.