Skip to main content
. 2009 Jul 29;83(19):10211–10223. doi: 10.1128/JVI.00571-09

TABLE 2.

Effect of temperature on binding parameters of MAb 2F5 with nominal and biepitope peptide-lipid conjugates

Parametera Binding of 2F5 at indicated temp to:
Nominal epitope
Biepitope
10°C 20°C 30°C 10°C 20°C 30°C
Rate constant
    ka1 (105M−1 s−1) 0.84 2.48 2.97 2.74 3.70 3.15
    kd1 (10−2 s−1) 16.6 8.30 9.80 4.66 8.81 13.6
    ka2 (10−2 s−1) 3.06 3.15 2.79 3.05 3.12 4.72
    kd2 (10−4 s−1) 5.28 1.24 1.48 0.20 2.85 4.48
Binding constant
    Kd (10−9 M) 0.65 1.30 1.75 0.11 2.16 4.07
    Kd1 (10−7 M) 1.21 3.34 3.30 4.63 2.38 4.31
    Kd2 (10−2) 0.55 0.39 0.53 0.05 0.92 0.95
Free-energy change
    ΔG (kcal) −12.10 −11.91 −12.13 −12.71 −11.62 −11.63
    ΔG1 (kcal) −9.11 −8.68 −8.99 −8.35 −8.88 −8.82
    ΔG2 (kcal) −2.98 −3.22 −3.16 −4.35 −2.73 −2.80
    %ΔG (ΔG2/ΔG) 25 27 26 34 23 24
a

ka1, ka2 for association and kd1, kd2 for dissociation processes in encounter and docking steps, respectively. Kd, Kd1, and Kd2 for overall reaction, encounter, and docking steps, respectively. ΔG, ΔG1 and ΔG2 for overall reaction, encounter, and docking steps, respectively.