Figure 7.
Oct-1 binding and transcription control is not affected by CM. A) Nuclear extracts from resting or 6 hr CM-stimulated DLD-1 cells were incubated with 32P-labeled oligonucleotides from Oct-1 motif at -10.2 kb of hiNOS promoter, followed by EMSA as described under Materials and Methods. B)A549 cells were transiently transfected with the same plasmids as those of Figure 6. Luciferase activities were determined 1 day after transfection in resting or 8 hr CM-stimulated A549 cells. (* indicates p<0.02 vs. empty vector. † indicates p<0.01 vs. pTK3XOct-1.)