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. Author manuscript; available in PMC: 2010 Jun 30.
Published in final edited form as: J Immunol Methods. 2009 Apr 15;345(1-2):49–59. doi: 10.1016/j.jim.2009.04.002

Fig. 1. Recombinant Human CD1-mouse IgG dimers associated with human β2-microglobulin.

Fig. 1

A) Schematic structures of human CD1 dimers, β2m/CD1-IgG dimer (left) and β2m-CD1-IgG dimer (right). α1, α2, α3 represent the extracellular domains of human CD1 molecules. The Flexible linker is the sequence (GGGSGGSGSGGGA) used to link β2m to the N-terminus of human CD1 molecules. B) SDS-PAGE and CBB staining of β2m/CD1-IgG and β2m-CD1-IgG dimers. Lane 1: recombinant human β2m (BD Biosciences, San Diego, CA, USA), 2: recombinant human CD1d dimer (BD Biosciences, San Diego, CA, USA), 3: β2m/CD1b-IgG dimer, 4: β2m/CD1c-IgG dimer, 5: β2m/CD1d-IgG dimer, 6: β2m-CD1b-IgG dimer, 7: β2m-CD1c-IgG dimer, 8: β2m-CD1d-IgG dimer. C) Western blotting with HRP-labeled anti-mouse IgG antibody. The lower bands are mouse immunoglobulin λ light chains produced by J558L cells and associated with CD1-immunoglobulin heavy chains. D) Western blotting with anti-human β2m antibody.