FIGURE 3.
ASC is required for zymosan- and mannan-induced caspase-1 activation and IL-1β secretion. A–C, BMDMs from wild type, ASC−/−, MyD88−/−, and RIP2−/− mice were left untreated (CTRL), or stimulated with zymosan, mannan, or LPS for 4 h, the last 30 min in the presence of 5 mm ATP. Cell extracts were immunoblotted for caspase-1 (A), and culture supernatants were analyzed for secreted IL-1β (B) and TNF-α (C). Black arrowheads indicate full-length caspase-1 (45 kDa), and white arrowheads mark the large subunits of activated caspase-1 (20 kDa). Cytokine data represent the mean ± S.E. of triplicate wells. All data are representative of three independent experiments.