Figure 3.
Confocal images showing neuronal differentiation of grafted NPs after transplantation of EGFP–NPs into the dentate gyrus of 7-d-old hippocampal slice cultures. After 3–7 d, some grafted EGFP–NPs (green, arrowhead in A) stained for NeuN (red, arrowhead in B and in merged image, yellow, in C). C, Three-dimensional analysis of double-fluorescent confocal image of NPs: EGFP (green) and anti-NeuN (red). In control slices, 9 of 230 EGFP-labeled cells were NeuN positive and 3 of 98 EGFP cells were NeuN positive in TNF-α–IFN-γ-injected slices. n = 3, 6 slices per group per experiment. Scale bars, 20 μm.