Figure 5.
MBH leucine activates Erk1/2 signaling pathway in MBH, PVN oxytocin, and DVC neurons. a–c, MBH (a), PVN (b), and DVC (c) Erk1/2 Thr202/Tyr204 phosphorylation and CREB Ser133 phosphorylation in mouse 30 min after an MBH injection of aCSF (n = 5) or leucine (n = 5) (injection design 1). d, Immunofluorescence showing colocalization (yellow, right) of oxytocin (Cy2, green, left) and MBH leucine-induced phospho-Thr202/Tyr204 Erk1/2 (Cy3, red, middle) in PVN slices of mice 30 min after MBH leucine administration (injection design 1).