Figure 3.
Leucine's anorexigenic effect requires direct activation of the melanocortin system. a, Sample traces recorded before and after application of l-leucine from a POMC neuron in current-clamp configuration. b, Immunofluorescence showing colocalization (yellow) of leucine-stimulated c-Fos-positive cells (Cy3, red) and arcuate POMC neurons of POMC-CRE Z/eGFP mice (green), 70 min after MBH leucine administration (injection design 1). c, Quantification of c-Fos-positive cells in different nuclei 70 min after MBH aCSF or leucine injection (injection design 1). d–f, First meal size (d), 12 h food intake (e), and 24 h change in body weight (BW) (f) in mouse (n = 8–12) after MBH coadministration of SHU9119 or aCSF and leucine or aCSF (injection design 3). Data are means ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.