FIGURE 7. CXCL16 expression increases 3H-Cholesterol release from Mϕ.
Mo were nucleofected with 150 nM of CXCL16-specific (CXCL16) or negative control (-Cmed) sRNAi and then cultured for 24 h with 50 µg/ml acLDL in the presence of radioactive cholesterol. Cells were equilibrated, washed and efflux was stimulated by incubating Mϕ in media containing lipid-poor acceptors for 6, 12 or 24 h. A, Macrophage 3H-Cholesterol efflux to human HDL. B, The release of 3H-Cholesterol to purified human ApoA-1. Results represent the mean ± SEM and are from 3 independent experiments using 3 different donors with each condition tested in triplicate. *p < 0.05, comparing the indicated value to the corresponding negative control sRNAi (-Cmed) value for the same time point.